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. Author manuscript; available in PMC: 2019 Sep 15.
Published in final edited form as: J Immunol. 2018 Jul 30;201(6):1775–1783. doi: 10.4049/jimmunol.1800649

FIGURE 4. AEC-PPARγ knockout increases expression of inflammatory and mucin genes.

FIGURE 4.

AAD was induced in WT and AEC-PPARγ−/− mice by OVA sensitization on days 0 and 7 and challenge on even-numbered days 14–22. Control mice were similarly treated with PBS. Twenty-four hours later, lung sections were prepared. AECs were collected from lung sections using laser capture microdissection as described in Materials and Methods. mRNA levels of indicated genes (Il25, Il33, Tslp, Ccl11 [eotaxin], Muc5ac, and Rela [NF-κB-p65]) were measured by real-time RT-PCR. The data are expressed as the mean ± SD with n = 3 mice/group. The results were reproduced at least two times independently; **P < 0.01, ***P < 0.001.