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. 2018 Sep 18;15:19. doi: 10.1186/s12950-018-0195-6

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Expression of IL-19, IL-20, IL-20R1, IL-20R2, IL-1β, TNF-α, and MCP-1 in DLS patients. Micrographs of IHC staining of cytokines and chemokines (IL-19, IL-20, IL-20R1, IL-20R2, IL-1β, TNF-α, and MCP-1) with specific antibodies on (a) Disc, (b) Facet joint, and (c) Ligamentum flavum tissue samples from patients with DLS (Disc, N = 6; Facet joint, N = 12; Ligamentum flavum, N = 13). Chondrocytes were positively stained. The boxed area shows positively stained fibrocytes and fibroblasts (original magnification ×200). Staining with isotype mouse IgG was used as the negative control. All experiments were performed three times with similar results. Data are from a representative experiment.