Table 1.
Overview of Laboulbeniomycetes sequences used in this study. Species names are listed for all isolates, with their hosts and country
| Genus | Species | Host | Country | Isolate | Extraction protocol | # thalli used | SSU | LSU |
|---|---|---|---|---|---|---|---|---|
| Arthrorhynchus | nycteribiae | Penicillidia conspicua | Hungary | Edeleny_ 13.xi.2014 | Heat extraction | 4–5 | KY094496 | KY094497 |
| Arthrorhynchus | nycteribiae | Penicillidia conspicua | Hungary | D. Haelew. 1015d | ExNA | 7 | MG438336 | MG438363 |
| Camptomyces | sp. nov. | Astenus sp. | Tanzania | D. Haelew. 1222d | REPLI‐g | 1 | MF314140 | MF314141 |
| Gloeandromyces | sp. nov. 3 | Trichobius joblingi | Panama | D. Haelew. 1312b | REPLI‐g, crushed | 2 | MH040546 | MH040580 |
| Gloeandromyces | sp. nov. 3 | Trichobius joblingi | Panama | D. Haelew. 1312c | REPLI‐g | 2 | MH040547 | MH040581 |
| Gloeandromyces | sp. nov. 3 | Trichobius joblingi | Panama | D. Haelew. 1323b | REPLI‐g, crushed | 4 | MG958011 | MH040582 |
| Gloeandromyces | sp. nov. 3 | Trichobius joblingi | Panama | D. Haelew. 1323c | REPLI‐g, crushed | 4 | MH040548 | MH040583 |
| Gloeandromyces | nycteribiidarum | Megistopoda aranea | Panama | D. Haelew. 1319b | REPLI‐g | 2 | MH040533 | MH040566 |
| Gloeandromyces | nycteribiidarum | Megistopoda aranea | Panama | D. Haelew. 1334c | REPLI‐g, crushed | 3 | MH040534 | MH040567 |
| Gloeandromyces | sp. nov. 1 | Trichobius joblingi | Panama | D. Haelew. 1306b | REPLI‐g | 2 | MH040541 | MH040574 |
| Gloeandromyces | sp. nov. 1 | Trichobius joblingi | Panama | D. Haelew. 1322a | REPLI‐g, crushed | 1 | MH040543 | MH040577 |
| Gloeandromyces | sp. nov. 1 | Trichobius joblingi | Panama | D. Haelew. 1327a | REPLI‐g, crushed | 1 | MH040544 | MH040578 |
| Gloeandromyces | sp. nov. 4 | Trichobius joblingi | Trinidad | D. Haelew. 619a | ExNA | 12 | MH040537 | KT800008 |
| Gloeandromyces | sp. nov. 4 | Trichobius joblingi | Panama | D. Haelew. 1073b | ExNA, prolonged, crushed | 3 | MH040538 | MH040570 |
| Gloeandromyces | sp. nov. 4 | Trichobius dugesioides | Panama | D. Haelew. 1089a | ExNA, prolonged, crushed | 4 | MH040539 | MH040571 |
| Gloeandromyces | sp. nov. 4 | Trichobius joblingi | Panama | D. Haelew. 1100b | ExNA, prolonged, crushed | 7 | MH040307 | MH040572 |
| Gloeandromyces | sp. nov. 3 | Trichobius dugesioides | Panama | D. Haelew. 1272a | REPLI‐g, crushed | 2 | MH040540 | MH040573 |
| Gloeandromyces | sp. nov. 4 | Trichobius joblingi | Panama | D. Haelew. 1315a | REPLI‐g, crushed | 1 | — | MH040575 |
| Gloeandromyces | sp. nov. 4 | Trichobius joblingi | Panama | D. Haelew. 1315b | REPLI‐g | 2 | MH040542 | MH040576 |
| Gloeandromyces | pageanus | Trichobius dugesioides | Panama | D. Haelew. 1091b | ExNA, prolonged, crushed | 6 | MH040535 | MG906798 |
| Gloeandromyces | pageanus | Trichobius dugesioides | Panama | D. Haelew. 1367b | EXNA, crushed, FastPrep | 6 | — | MH040568 |
| Gloeandromyces | pageanus | Trichobius dugesioides | Panama | D. Haelew. 1425a | REPLI‐g, crushed | 4 | MH040536 | MH040569 |
| Gloeandromyces | streblae | Trichobius dugesioides | Panama | D. Haelew. 1090a | ExNA, prolonged, crushed | 7 | — | MH040584 |
| Gloeandromyces | streblae | Trichobius joblingi | Panama | D. Haelew. 1306c | REPLI‐g | 4 | MG958012 | MH040585 |
| Gloeandromyces | streblae | Trichobius dugesioides | Panama | D. Haelew. 1308b | REPLI‐g | 2 | MH040549 | MH040586 |
| Gloeandromyces | streblae | Trichobius dugesioides | Panama | D. Haelew. 1309a | REPLI‐g | 1 | MH040550 | MH040587 |
| Gloeandromyces | streblae | Trichobius joblingi | Panama | D. Haelew. 1317a | REPLI‐g | 1 | MH040551 | MH040588 |
| Gloeandromyces | streblae | Trichobius joblingi | Panama | D. Haelew. 1335c | REPLI‐g, crushed | 2 | MH040552 | MH040589 |
| Gloeandromyces | sp. nov. 2 | Trichobius joblingi | Panama | D. Haelew. 1320b | REPLI‐g, crushed | 1 | MH040545 | MH040579 |
| Herpomyces | chaetophilus | Periplaneta americana | USA | D. Haelew. 483b | ExNA | 11 fem | MG438319 | MG438350 |
| Herpomyces | chaetophilus | Periplaneta americana | USA | D. Haelew. 602b | ExNA | 10 fem | KT800023 | KT800009 |
| Herpomyces | periplanetae | Periplaneta americana | USA | D. Haelew. 602d | ExNA | 8 fem | MG438327 | MG438357 |
| Herpomyces | periplanetae | Periplaneta americana | USA | D. Haelew. 1187d | REPLI‐g | 1 fem | MG438331 | MG438359 |
| Herpomyces | shelfordellae | Shelfordella lateralis | Hungary | DE_HerpBL1 | Heat extraction | ±30 | KT800026 | KT800011 |
| Herpomyces | shelfordellae | Shelfordella lateralis | Hungary | Bud_Slat | Heat extraction | 10–20 | MG438333 | MG438361 |
| Herpomyces | stylopygae | Blatta orientalis | Hungary | Bud_Bori | Heat extraction | 10–20 | MG438332 | MG438360 |
| Hesperomyces | coleomegillae | Coleomegilla maculata | Ecuador | 631C | 0.1 × TE buffer + dry ice | 3–15 | KF266882 | — |
| Hesperomyces | coleomegillae | Coleomegilla maculata | Ecuador | 632A | 0.1 × TE buffer + dry ice | 3–15 | KF266880 | — |
| Hesperomyces | palustris | Coleomegilla maculata | Ecuador | 631K | 0.1 × TE buffer + dry ice | 3–15 | KF266902 | — |
| Hesperomyces | palustris | Coleomegilla maculata | Ecuador | 632B | 0.1 × TE buffer + dry ice | 3–15 | KF266891 | — |
| Hesperomyces | virescens | Harmonia axyridis | USA | D. Haelew. 316a | ExNA | 10–12 | MG438339 | KJ842339 |
| Hesperomyces | virescens | Harmonia axyridis | Netherlands | D. Haelew. 334b | ExNA | 10 | MG438340 | MG438364 |
| Hesperomyces | virescens | Olla v‐nigrum | USA | JP352b | ExNA | 11 | MG760581 | MG745337 |
| Hesperomyces | virescens | Olla v‐nigrum | USA | JP353a | QIAamp Micro | 10 | KT800028 | KT800013 |
| Hesperomyces | virescens | Olla v‐nigrum | USA | JP354b | ExNA | 10 | MG760583 | MG745339 |
| Hesperomyces | virescens | Harmonia axyridis | South Africa | D. Haelew. 648c | ExNA | 8–10 | KU574863 | KU574865 |
| Hesperomyces | virescens | Cheilomenes propinqua | South Africa | D. Haelew. 655c | ExNA | 11 | KU574866 | KU574867 |
| Hesperomyces | virescens | Cheilomenes propinqua | South Africa | D. Haelew. 659a/b | ExNA | 20 | MG760590 | MG745342 |
| Hesperomyces | virescens | Harmonia axyridis | Netherlands | D. Haelew. 1174a | ExNA, crushed, prolonged | 12 | MG760598 | MG745345 |
| Hesperomyces | virescens | Adalia bipunctata | Denmark | D. Haelew. 1193g | REPLI‐g, crushed | 1 | MG760599 | MG745346 |
| Hesperomyces | virescens | Adalia bipunctata | Sweden | D. Haelew. 1199h | REPLI‐g, crushed | 1 | MG760600 | MG745347 |
| Hesperomyces | virescens | Olla v‐nigrum | USA | D. Haelew. 1200i | REPLI‐g, crushed | 4 | MG760602 | MG745349 |
| Hesperomyces | virescens | Adalia bipunctata | Italy | D. Haelew. 1231a | REPLI‐g | 2 | MG760603 | MG745350 |
| Hesperomyces | virescens | Psyllobora vigintimaculata | USA | D. Haelew. 1250b | REPLI‐g | 5 | MG760607 | MG745354 |
| Hesperomyces | virescens | Psyllobora vigintimaculata | USA | D. Haelew. 1250c | REPLI‐g, crushed | 2 | MG760608 | MG745355 |
| Hesperomyces | virescens | Psyllobora vigintimaculata | USA | D. Haelew. 1251b | REPLI‐g, crushed | 1 | MG760609 | MG745356 |
| Hesperomyces | virescens | Harmonia axyridis | Japan | D. Haelew. 1268b | REPLI‐g, crushed | 3 | MG760610 | MG745357 |
| Nycteromyces | streblidinus | Trichobius parasiticus | Honduras | D. Haelew. 956a | ExNA | 8 fem | MH040553 | — |
| Nycteromyces | streblidinus | Trichobius joblingi | Panama | D. Haelew. 1324b | REPLI‐g, crushed | 4 m | MH040554 | MH040590 |
| Nycteromyces | streblidinus | Trichobius joblingi | Panama | D. Haelew. 1324c | REPLI‐g, crushed | 1 fem | MH040555 | — |
| Nycteromyces | streblidinus | Trichobius joblingi | Panama | D. Haelew. 1324d | REPLI‐g | 1 fem | MH040556 | MH040591 |
| Nycteromyces | streblidinus | Trichobius joblingi | Panama | D. Haelew. 1324e | REPLI‐g, crushed | 1 m | MH040557 | MH040592 |
| Polyandromyces | coptosomalis | Phoeacia sp. nov. | Ecuador | D. Haelew. 313f | ExNA | 7 fem, 2 m | KT800035 | KT800020 |
| Polyandromyces | coptosomalis | Acrosternum sp. | Canary Islands | HM499a | ExNA | 15 fem, 3 m | MG438347 | — |
| Prolixandromyces | triandrus | Velia (Plesiovelia) saulii | Hungary | Nagyvisnyo1 | Heat extraction | 5 | LT158294 | LT158295 |
| Rickia | laboulbenioides | Cylindroiulus punctatus | Denmark | SR4s | ExNA, crushed | 5 | MH040558 | MH040593 |
| Rickia | pachyiuli | Pachyiulus hungaricus | Serbia | SR1s | ExNA, crushed | 10–12 | MH040559 | MH040594 |
| Rickia | wasmannii | Myrmica scabrinodis | Hungary | DE_Rak4 | Heat extraction | 30 | KT800037 | KT800021 |
| Rickia | wasmannii | Myrmica sabuleti | Netherlands | D. Haelew. 1234a | REPLI‐g | 3 | MH040560 | MH040595 |
| Stigmatomyces | borealis | Parydra breviceps | USA | AW‐797 | JN835186 | — | ||
| Stigmatomyces | ceratophorus | Fannia canicularis | USA | D. Haelew. 1136h | REPLI‐g, crushed | 8 | MG958013 | MH145384 |
| Stigmatomyces | entomophilus | Drosophila funebris | Netherlands | D. Haelew. 1062c | ExNA, prolonged, crushed | 6 | MG958014 | — |
| Stigmatomyces | entomophilus | Drosophila funebris | Netherlands | D. Haelew. 1063a | ExNA, prolonged, crushed | 14 | MH040561 | — |
| Stigmatomyces | gregarius | Diopsidae sp. | Sierra Leone | D. Haelew. 1008a | ExNA | 5 | MG438348 | — |
| Stigmatomyces | gregarius | Diopsidae sp. | Sierra Leone | D. Haelew. 1008b | ExNA | ±10 | MH040562 | — |
| Stigmatomyces | hydrelliae | Hydrellia sp. | 0.1 × TE buffer + dry ice | 4–10 | AF431757 | — | ||
| Stigmatomyces | limnophorae | Muscidae sp. | USA | AW‐785 | 1% Triton 100 | 4–10 | AF407576 | — |
| Stigmatomyces | protrudens | Ephydridae sp. | USA | AW‐793 | 0.1 × TE buffer + dry ice | 4–10 | AF298232 | AF298234 |
| Stigmatomyces | rugosus | Psilopa sp. | 0.1 × TE buffer + dry ice | 4–10 | AF431759 | — | ||
| Stigmatomyces | rugosus | Psilopa sp. | Portugal | D. Haelew. 1138a | ExNA, prolonged, crushed | 6 | MH040563 | — |
| Stigmatomyces | scaptomyzae | Scaptomyza sp. | 0.1 × TE buffer + dry ice | 4–10 | AF431758 | — | ||
| Stigmatomyces | sp. nov. | cf. Chamaemyia | Portugal | D. Haelew. 1137a | ExNA, prolonged, crushed | 8 | MH040564 | — |
| Stigmatomyces | sp. nov. | cf. Chamaemyia | Portugal | D. Haelew. 1137c | ExNA, prolonged, crushed | 1 | MH040565 | — |
Notes. Also included are extraction protocols and numbers of thalli used per extraction for all isolates: 1% Triton 100‐based protocol from Weir and Blackwell (2001a); 0.1 × TE buffer + dry ice protocol from Weir and Blackwell (2001b); heat extraction protocol, Extract‐N‐Amp Plant PCR Kit (ExNA) and QIAamp DNA Micro Kit (QIAamp Micro) from Haelewaters et al. (2015); REPLI‐g Single Cell Kit (REPLI‐g) from Haelewaters et al. (in review). GenBank accession numbers are provided (newly generated sequences in bold).