Fig. 6.

LncRNA SNHG5/miR-212-3p/SGK3 axis regulate the progression of osteosarcoma. a, b Transwell and MTT assays were performed to detect cell growth, migration, and invasion abilities after SGK3 silencing. c The protein expression of E-cadherin, N-cadherin, Vimentin and β-cantenin were determined by western-blotting analysis. d qPCR detected the expression of ZEB1, TWIST. e qPCR detected the expression of Bad, Bim, Bcl-xl, AIP4, FLII, GSK3β after transfection of mimics, si-SGK3, si-SNHG5. f Transwell assays were performed to detect the cell migration and invasion abilities after SGK3 knockdown, which was rescued by pcDNA-SNHG5 and miR-212-3p inhibitor treatment. n = 3, **P < 0.01, compared to NC, ## P < 0.01, inhibition vs. si-SGK3+inhibition, && P < 0.01, pcDNA-SNHG5 vs. si-SGK3+pcDNA-SNHG5