Figure 2.
TMEM119 was highly expressed in gastric cancer cell lines. TMEM119 mRNA and protein expression in gastric cancer cell lines, including SGC-7901, AGS, and MKN45, and gastric epithelial cell line GES-1 was measured by real-time PCR (A) and Western blotting (B and C), respectively. TMEM119 mRNA and protein expression in MKN45 cells with recombined pLVX-Puro-TMEM119 vector transfection was measured by real-time PCR (D) and Western blotting (E and F). **P<0.01 compared with GES-1 cells or vector.
Abbreviation: PCR, polymerase chain reaction.