Figure 4.

Oscillatory strain affects vessel development and stabilization through secretion of pro‐angiogenic cytokines, fibroblasts differentiation, and collagen VI production. A) Cytokine secretion array was performed on medium collected from oscillatory stretched and static control constructs at days 7, 14, and 21 of culture. Pro‐angiogenic factors were secreted over longer time periods under oscillatory stretch conditions. B) αSMA, PDGFRβ, NG2, and collagen 4 staining of oscillatory stretched and static control constructs cultured for 14 d. C–E) αSMA, PDGFRβ, and NG2 colocalization with ECs vessels quantification. F–I) Markers and blood vessels alignment quantification. Scale bar = 10 µm; *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.