Fig. 2.

Distribution of DiI labeled cells after cranial neural crest transplantation. (A–I) Transplantation from surface fish donors into surface fish hosts. (J–U) Transplantation from surface fish donors into cavefish hosts. (A–Q) Each pair of bright field and fluorescence images shows four different host individuals that received a neural crest graft at 2 dpf and were assayed at 4 dpf. (Ia–c) Trunk region of the individual shown in G, H with arrows indicating DiI labeled cells. Posterior is on the right in each frame. (R–U) The same cavefish host viewed from the right (R, S) and left (T, U) sides. (V–Y) Transplantation from cavefish donors into cavefish hosts. Each pair of bright field and fluorescence images shows two different host individuals that received a neural crest graft at 1 dpf and were assayed at 4 dpf. e, eye; n, nasal area; o, otic area. Scale bar: 200 µm. Bright field images: A, C, E. G, I-a, J, L, N. P, R, U, V, X. Fluorescence images: B, D, F, H, I-b, K, M, O, Q, S, T, W, Y. Merged images of I-a and I-b: I-c.