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. Author manuscript; available in PMC: 2019 Aug 21.

Published in final edited form as: J Am Coll Cardiol. 2018 Aug 21;72(8):885–904. doi: 10.1016/j.jacc.2018.05.061

Figure 3. — LVs from the 2 mouse genotypes before and at the indicated days after MI were digested, stained with appropriate antibodies, and analyzed by flow cytometry to determine monocyte and macrophage phenotypes. Each panel depicts a specific cell subset based on the expression of cell markers. (A) neutrophils (left) and total macrophages (right); (B) total CCR2⁺ (left) and CCR2⁻ macrophage subsets (right); (C) total Ly6C^hi (left) and total Ly6C^lo macrophage subsets (right); (D) CCR2⁺/Ly6C^hi (left) and CCR2⁻/Ly6C^hi macrophage subsets (right); (E) CCR2⁺/Ly6C^lo (left) and CCR2⁻/Ly6C^lo macrophage subsets (right). Each data point represents 1 mouse/genotype. Total CCR2⁺, CCR2⁻, Ly6C^hi, or Ly6C^lo cells were calculated by addition of the frequencies of each cell subset. Data from the 2 groups (Cre versus mGATA3KO) before and after MI were analyzed by Student t-test. Each data point represents 1 mouse; therefore, the number of animals in each group is equal to the number of dots/group. Abbreviations as in Figure 1.

Figure 3. — LVs from the 2 mouse genotypes before and at the indicated days after MI were digested, stained with appropriate antibodies, and analyzed by flow cytometry to determine monocyte and macrophage phenotypes. Each panel depicts a specific cell subset based on the expression of cell markers. (A) neutrophils (left) and total macrophages (right); (B) total CCR2⁺ (left) and CCR2⁻ macrophage subsets (right); (C) total Ly6C^hi (left) and total Ly6C^lo macrophage subsets (right); (D) CCR2⁺/Ly6C^hi (left) and CCR2⁻/Ly6C^hi macrophage subsets (right); (E) CCR2⁺/Ly6C^lo (left) and CCR2⁻/Ly6C^lo macrophage subsets (right). Each data point represents 1 mouse/genotype. Total CCR2⁺, CCR2⁻, Ly6C^hi, or Ly6C^lo cells were calculated by addition of the frequencies of each cell subset. Data from the 2 groups (Cre versus mGATA3KO) before and after MI were analyzed by Student t-test. Each data point represents 1 mouse; therefore, the number of animals in each group is equal to the number of dots/group. Abbreviations as in Figure 1.