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. Author manuscript; available in PMC: 2019 Aug 21.

Published in final edited form as: J Am Coll Cardiol. 2018 Aug 21;72(8):885–904. doi: 10.1016/j.jacc.2018.05.061

Figure 4. — Peripheral blood was collected from the 2 mouse genotype groups before and at the indicated days after MI, stained with the appropriate antibodies, and analyzed by flow cytometry, essentially as described in Figure 3 using the gating strategy outlined in Online Figure 4. Each panel depicts a specific cell subset based on the expression of cell markers; (A) Left: CCR2⁺/Ly6C^hi monocytes; right: CCR2⁻/Ly6C^hi monocytes. (B) Left: total Ly6C^lo monocytes; right: CCR2⁻/Ly6C^lo monocyte subset. Each data point represents a blood sample from an individual mouse. The number of animals in each group is equal to the number of dots/group. MI = myocardial infarction; NS = not significant.

Figure 4. — Peripheral blood was collected from the 2 mouse genotype groups before and at the indicated days after MI, stained with the appropriate antibodies, and analyzed by flow cytometry, essentially as described in Figure 3 using the gating strategy outlined in Online Figure 4. Each panel depicts a specific cell subset based on the expression of cell markers; (A) Left: CCR2⁺/Ly6C^hi monocytes; right: CCR2⁻/Ly6C^hi monocytes. (B) Left: total Ly6C^lo monocytes; right: CCR2⁻/Ly6C^lo monocyte subset. Each data point represents a blood sample from an individual mouse. The number of animals in each group is equal to the number of dots/group. MI = myocardial infarction; NS = not significant.