Figure 1. Growth inhibitory activity of JIB-04 in Ewing Sarcoma.

(A) One day following plating, the indicated cells (7 different Ewing Sarcoma cell lines, and human mesenchymal stem cells (hMSC)) were treated for 48 hours with the indicated concentrations of JIB-04. Cell numbers at the end of the experiment were quantified using an MTT assay, and were normalized to vehicle-treated cells. Results represent the mean and standard error of the mean (SEM) of at least 2 independent experiments, each performed in replicate. IC50 values for growth/survival inhibition of Ewing Sarcoma cells by JIB-04, calculated from the data in panel A, are shown on right. (B) Beginning one day following plating (500 cells per well), cells were treated with the indicated concentrations of JIB-04 (or vehicle control) every 2 days. Colonies were visualized by crystal violet staining approximately 2 weeks later. Representative images of triplicate platings are shown.