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. 2018 Sep 4;9(69):33110–33123. doi: 10.18632/oncotarget.26011

Figure 2. Effects of JIB-04 on global histone methylation in Ewing Sarcoma cells.

Figure 2

Levels of the indicated histone methyl marks, in vehicle and JIB-04-treated cells (TC32: 0.3 μM; SK-ES-1 and SK-N-MC: 1 μM; A673: 2 μM), as determined by immunoblotting of acid-extracted histones collected at 36 hours following treatment. Representative blots at the top. Shown below is densitometric quantification of histone methyl marks levels, normalized to total histone H3 levels, and plotted relative to vehicle-treated cells (mean and SEM of 2-3 independent experiments, each done in duplicate).