Fig. 2.

Slamf9 is expressed in murine melanoma-derived TAM. a 1 × 10⁶ B16F1 cells were injected subcutaneously into the flank of C57BL/6 wild-type mice. The tumors were excised 14 days after the injection. Tumors were homogenized and cells were separated according to CD11b expression by magnetic cell sorting. The expression of Slamf members was assessed by qRT-PCR analysis. Values are normalized to an internal ß-actin control (n = 3). Data are presented as mean ± SEM. b Western blot analysis of three independent clones of transgenic Slamf9⁺ RAW 264.7 cells and EV RAW 264.7 cells using the self-generated monoclonal anti-mmSlamf9 antibody, rat IgG1 isotype control or anti-GAPDH antibody. c Immunohistological staining of acetone-fixed cryo-sections of murine B16F1 tumors using our self-generated monoclonal anti-mmSlamf9 antibody or rat IgG1 isotype control. One representative picture is shown, scale bar = 100 µm