Fig. 3.

Slamf-9 is induced in BMDM by stimulation with IFN-γ. After 4 days of stimulation with M-CSF, medium of BMDM was exchanged by new M-CSF containing culture medium supplemented with different combinations of pro- and anti-inflammatory mediators as indicated for three further days. The gene expression of Slamf members was examined by qRT-PCR. Normalization was performed based on an internal ß-actin control. a Summary of significant mRNA upregulation of Slamf members stimulated as indicated and compared to M-CSF stimulated BMDM (n = 3). b Bar chart of the relative gene expression of Slamf9 mRNA (normalized to ß-actin) assessed by qRT-PCR (n = 3). Data are presented as mean ± SEM