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. 2018 Sep 19;8:14026. doi: 10.1038/s41598-018-32399-x

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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UV-Vis spectrum analysis and visual colors of HEBR fusion proteins. Four separate measurements of absorbance spectra were plotted in the same chart: solely HEBR (bold dashed), target protein (dashed) and fusion protein (solid): (a) HEBR-HsBR, (b) HEBR-NpHR, (c) HEBR-HmSRII and (d) HEBR-HmSRM. The visual color of purified proteins (inlet of their corresponding figure). All the samples were prepared in 50 mM MES, 4 M NaCl, pH 5.8, containing 0.05% DDM.