Table 1.
Primer sequences and probes used in this study.
| Genes | Forward (5′–3′) | Reverse (5′–3′) | Probe |
|---|---|---|---|
| IL-6 | CTGCAAGTGC ATCATCGTTGT |
TGTCTATACCAC TTCACAAGTCGGA |
CAGAATTGCCATTGC ACAACTCTTTTCTCA |
| TNF-α | TGTCTACTGAAC TTCGGGTGAT |
AACTGATGA GAGGGAGGCCAT |
TCCCCAAAGGGAT GAGAAGTTCCCAA |
| TLR4 | CCATGCCATGCC TTGTCTT |
GAGCTTCAACCC CTTGAAGATCT |
TCAATTTCACACC TGGATAAATCCAGCCA |
| NF-κB | TGTCTGCCTCTCTCG TCTTCCT |
CACCACTGTCACA GACGCTGT |
CGAGGCAGCACATAG ATGAACTCCGG |
| 18S | ATGAGTCCACTT TAAATCCTTTAACGA |
CTTTAATATACGC TATTGGAGCTGGAA |
ATCCATTGGAGG GCAAGTCTGGTGC |
| CCL2 | GTTGGCTCAGCC AGATGCAG |
GTAGCTCTCCAGCC TACTCATTG |
CCCACTCACCTGC TGCTACTCATTCACC |
| HO-1 | CAGGGTGACAGA AGAGGCTAAGAC |
TTGTGTTCCTCTG TCAGCATCAC |
TCCTGCTCAACATT GAGCTGTTTGAGGA |
| Sirt1 | CAGCATCTTGCCTG ATTTGTAAATAC |
CACCGAGGAACTA CCTGATTAAAAA |
TCTCCACGAACAGC TTCACAATCAACT |
| NLRP3 | AGGACCCACAGTGT AACTTGC |
CAGAGGTCAGAGC TGAACAACA |
TAAGGCCGGAATTC ACCAACCCCAGCT |