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. 2018 Sep 19;8:14038. doi: 10.1038/s41598-018-32335-z

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Autoblinking levels in D. deserti, D. radiodurans, B. subtilis and E. coli. (a) Number of localizations per μm² per 1000 frames extracted from images acquired with 50 ms exposure under continuous 0.8 kW/cm² 561 nm laser. Individual data points correspond to the autoblinking levels derived from a given stack of images. Means and standard deviations are plotted in the graph. Inset: examples of raw autoblinking signal (bright white spots) observed in the cell periphery of a D. radiodurans tetrad (outlined in red and presented in Fig. 2) at different timepoints during image acquisition (see also Supplementary Movie S1). Scale bar: 1 μm. (b) Representative reconstructions of live, unlabeled D. deserti (1), D. radiodurans (2), B. subtilis. (3) and E. coli (4) superimposed on their respective brightfield images. In each case, the reconstructed images are derived from a stack of 1000 frames of 50 ms exposure acquired under continuous 0.8 kW/cm² 561 nm laser. Scale bar: 2 μm.