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. 2018 Sep 19;8:14038. doi: 10.1038/s41598-018-32335-z

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Two-color PALM/PAINT imaging of D. radiodurans. (a–c) PALM/PAINT imaging of D. radiodurans cells expressing cytoplasmic PAmCherry. (a) Autoblinking-based PAINT image (50 ms frametime; 561 nm laser only), superimposed on the brightfield image. (b) PAmCherry-based PALM image (4.8 ms frametime; 561 nm plus 405 nm lasers). (c) Superimposed image of (a) and (b). (d–f) PALM/PAINT imaging of D. radiodurans cells expressing PAmCherry fused to HU. (d) Autoblinking-based PAINT image (50 ms frametime; 643 nm laser), superimposed on the brightfield image. (e) PAmCherry-based PALM image (50 ms frametime; 561 nm laser). (f) Superimposed image of (d) and (e). Scale bar: 1 μm.