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. 2018 Sep 19;8:14038. doi: 10.1038/s41598-018-32335-z

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Cell division and septa formation in D. radiodurans. (a) Autoblinking-based PAINT image (15 ms frametime; 561 nm laser) of exponentially growing, live, unlabeled D. radiodurans cells. Bacteria are seen at different stages of their division cycle shown schematically in (b). Scale bar: 1 μm. Inset: close-up view of a D. radiodurans diad (indicated with a *) in which the double-layered cell wall separating the two bacteria can clearly be seen. (b) Schematic representations of D. radiodurans morphology along its cell cycle. Representative bacteria in each stage are shown in red as Voronoï diagrams following cluster analysis of the image shown in (a). Identified clusters are highlighted in yellow. (c) Number of autoblinking molecules per cluster as a function of cluster area. (d) Individual tracks of autoblinking molecules superimposed on the corresponding brightfield image. The faster and slower diffusing molecules are shown in red and blue respectively.