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. 2018 Sep 19;9:3814. doi: 10.1038/s41467-018-06362-3

Fig. 1.

Fig. 1

Schematic of single-molecule imaging of SmChiA probed by gold nanoparticle. a SmChiA has a flat surface for binding to crystalline chitin and the cleft for threading decrystallized single chain of chitin. b A single AuNP was attached to SmChiA via streptavidin-biotin interaction. Elementary steps were categorised as chemical (substrate-assisted catalysis and product release) or mechanical (decrystallization and chain sliding) step. Length of the reaction product chitobiose (1.04 nm) corresponds to expected step size of SmChiA movement