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. 2018 Sep 19;9:3814. doi: 10.1038/s41467-018-06362-3

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — Stepping movement of SmChiA probed by 40-nm AuNP. a Example of image sequence. Scale bar is 400 nm. b Trajectory of SmChiA movement shown in a. Raw trajectory: light green; median-filtered (current ± 2 ms): dark green. Magenta line: steps and pauses fitted to median-filtered trajectory. Two-dimensional trajectory of non-moving molecule in the same field of view is also shown, in the same scale. c Examples of expanded trace. Forward, backward, and recovery step sizes and dwell times before these steps are indicated. Scale bars on vertical and horizonal axes are 1 nm and 30 ms, respectively. d Forward and backward-step size distribution. The peak values of Gaussian fittings (blue line) are shown. Step ratios were estimated from Gaussian areas. e On-axis localisation precision during pauses. The value is peak ± SD of Gaussian fitting (blue line)