Fig. 3.

Dwell time analysis of SmChiA movement. a, b Dwell time distributions before 1 nm forward steps in H₂O (a) and D₂O (b) fitted by a model assuming consecutive reaction with two time constants (blue lines). c–e Dwell time distributions before backward (c), recovery (d), and before > 2 nm forward (e) steps fitted by single exponential decays (blue lines). The values are obtained parameters ± fitting errors. f Time constant summary of processive reaction cycle. Equilibrium constant between forward (state A) and backward (state E) stepped states is 1.1. Probabilities of substrate-assisted catalysis (86.3%) and backward step (13.7%) from state A are consistent with probabilities estimated by event number in Fig. 2d