. Author manuscript; available in PMC: 2019 Sep 19.

Published in final edited form as: J Am Chem Soc. 2018 Sep 10;140(37):11777–11783. doi: 10.1021/jacs.8b07277

Table 2.

Ground state parameters of the different αKG only and αKG+CAD bound complexes of the D201X variants. *All values in cm⁻¹. VTVH parameters for the wt enzyme are taken from ref. 19.

Variant	δ*	^g\|\|	Δ*	\|V\|*
Fe^II Sites
wt-FIH	3.9	9.2	−275	100
Fe^II/αKG Sites
wt-FIH	2.8	8.9	−950	530
D201GCl	2.5	9.05	−950 ± 100	475
D201G	5.0	8.0	950 ± 40	210
D201A	5.0	8.0	700 ± 80	330
D201E	2.4	8.9	−1100 ± 60	570
Fe^II/αKG/CAD Sites
wt-FIH	1.8	9.2	−1200	410
D201GCl	3.7	8.0	1150 ± 50	480
D201G	4.0	8.0	1000 ± 80	340
D201A	5.3	8.0	700 ± 40	360