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. 2018 Sep 13;12:72. doi: 10.3389/fnana.2018.00072

FIGURE 6.

FIGURE 6

Luminal assemblies of macromolecules in DCVs and SVs. (A) A 3 nm thick virtual slice of a docked DCV, and (B) a 1 nm thick virtual slice of a docked SV showing the luminal and cytosolic surfaces of the vesicle membrane (outlined in blue) and the luminal assembly of macromolecules (outlined in orange). (C) A surface model from 10 virtual slices including the one in (A). (D) A surface model from 35 virtual slices including the one in (B). The sites where nubs link the luminal assemblies to the vesicle membrane are blue. The luminal assembly in the DCV comprises the dense core. The DCV is from a muscle fixed with an aqueous solution of glutaraldehyde and stained with aqueous solutions of osmium tetroxide and uranyl acetate. The SV is from a muscle fixed by rapid freezing and stained by freeze-substitution of osmium tetroxide and uranyl acetate in acetone. (B,D) Taken from Harlow et al. (2013). Scale bar = 50 nm.