Figure 2.

Relationship between lipid stores, insulin secretion, and insulin content in INS1 (832/13) cells cultured in the presence of different nutrients: 1, 4 mM glucose for more than 2 weeks (chronic) (n = 12); 2, 4 mM glucose for 48 h (n = 8); 3, 4 mM glucose chronic + 0.15 mM oleate for 24 h (n = 6); 4, 11 mM glucose (n = 13); 5, 11 mM glucose + 0.15 mM oleate for 48 h (n = 4). As standard culture media glucose concentration for INS1 cells is 11 mM, cells identified as being cultured in 4 mM glucose were switched from 11 to 4 for the stated period of time. Lipid content was measured using Nile Red. (A) Relationship between Insulin secretion at 4 mM glucose following washout of growth media and lipid stores. (B) Correlation of intracellular lipid stores and insulin secretion at 12 mM glucose. (C) Relationship between lipid stores and total insulin content. Data in this figure were recalculated from previously published results (7).