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Optimization of Arabidopsis germination HTS. Correlation between the (A) number of seeds per well and the absorbance under control, moderate (75 mM NaCl), and severe (150 mM NaCl) stress conditions, (B) manually counted number of seeds and the software-estimated number of seeds per well, and (C) germination rate (%) and the estimated number of seeds. The equation of the curve and the Pearson’s correlation coefficient with significance according to ANOVA were calculated. ∗∗∗p < 0.001.
