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. 2018 Sep 13;8:321. doi: 10.3389/fcimb.2018.00321

Figure 4.

Figure 4

PMN-like MDSC levels are modulated by heat-stable S. aureus proteins sized 10 to 50 kDa. S. aureus supernatants were pre-treated prior to stimulation of PBMC. (A) Pre-treatment with 20 μg/ml of proteinase K at 37°C for 16 h, (B) heat-treatment at 80°C for 20 min, (C) filtration through spin columns with MWCO cut-off pores as indicated. 3% (USA300 or Newman) or 10% (NCTC 8325) of supernatants were used as high concentrations (black bars), 0.01% (all tested strains) were used as low concentrations (gray bars). Data are presented as means ± SEM. Differences between stimulated samples and medium controls were analyzed by a one-sample t-test. Differences between different pre-treatment conditions (A,B) were analyzed by a Mann-Whitney test. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001; ns–not significant.