Figure 7.

S. aureus supernatants and enterotoxins interfere with other MDSC-inducing substances. PBMC were stimulated simultaneously with 10 ng/ml GM-CSF and increasing concentrations of Newman supernatants (A) or SEA (B). High concentrations of S. aureus supernatants (3%) or enterotoxins (5 ng/ml) were used for co-stimulation with IL-2 (100 U/ml) (C), P. aeruginosa PAO-1 supernatants (1%) (D) or Aspergillus fumigatus lysates (10 μg/ml) (E). Data represent means ± SEM. Differences between stimulated PBMC and medium controls (white bars) were analyzed by a one-sample t-test. Differences between single and mixed stimulations were analyzed by a Mann-Whitney test *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001; ns–not significant.