Table 1.
Bacterial strains used in this study.
| Strain (substrain) | Strain ID [a] | Genotype and description [b] | References |
|---|---|---|---|
| S. aureus | |||
| USA300 (FPR3757) | ATCC BAA-1516, NCBI 451515, NRS482 | Referred to as ‘USA300’ in this study; CA-MRSA; agr+ | McDougal et al., 2003; Diep et al., 2006 |
| USA300 (LAC) Δagr | n.a. | CA-MRSA; deleted agr locus | Cheung et al., 2011 |
| USA300 (JE2) | JE2 | CA-MRSA; derived from USA300 (LAC) by curing of three plasmids; parental strain of transposon insertion mutants collected in the Nebraska Transposon Mutant Library | Fey et al., 2013 |
| USA300 (JE2) secA- | NE66 | Tn insertion in preprotein translocase gene SAUSA300_2584 | Fey et al., 2013 |
| USA300 (JE2) putative enterotoxin type A- | NE309 | Tn insertion in putative enterotoxin type A gene SAUSA300_1559 | Fey et al., 2013 |
| USA300 (JE2) sek- | NE1255 | Tn insertion in enterotoxin K gene SAUSA300_0800 | Fey et al., 2013 |
| USA300 (JE2) seq- | NE1605 | Tn insertion in enterotoxin Q gene SAUSA300_0801 | Fey et al., 2013 |
| USA300 (JE2) selX- | NE1809 | Tn insertion in putative enterotoxin selX gene SAUSA300_0370 | Fey et al., 2013 |
| USA300 (SF8300) | n.a. | CA-MRSA | Diep et al., 2008b |
| USA300 (SF8300) ΔACME | n.a. | Deleted ACME locus | Diep et al., 2008b |
| USA400 (MW2) | ATCC BAA-1707, NCBI 196620, NRS123 | CA-MRSA | Baba et al., 2002 |
| N315 | NCBI 158879, NRS70 | HA-MRSA | Kuroda et al., 2001 |
| Mu50 | ATCC 700699, NCBI 158878, NRS1 | Vancomycin-intermediate HA-MRSA | Kuroda et al., 2001 |
| COL | NCBI 93062, NRS100 | HA-MRSA, agr low, sigB+ | Gill et al., 2005 |
| Newman | ATCC 13420, NCTC 8178, NCBI 426430 | MSSA; agr+; saeRS constitutively expressed; PVL- phenotype; fnbA- and fnbB- | Baba et al., 2008 |
| Newman φSa2MW | n.a. | Newman lysogenized with φSa2MW carrying lukF/S-PV | Wirtz et al., 2009 |
| PS187 | ATCC 15564, NCTC 9754, NCBI 1323662 | MSSA; prototype of ST395 lineage; unique WTA structure with a GroP-GalNAc backbone | Winstel et al., 2013 |
| NCTC 8325; RN1 | NCTC 8325, NCBI 93061, NRS77 | MSSA; parental strain of 8325-4, RN4220, HG003, SA113, RN6390; φ11+ φ12+ φ13+, agr+, rsbU-, tcaR- | Novick, 1967 |
| HG003 | n.a. | RN1 derivative; φ11+ φ12+ φ13+, agr+, rsbU and tcaR repaired | Herbert et al., 2010 |
| SA113 | ATCC 35556, DSM 4910 | RN1 derivative; MNNG mutagenesis; φ11+ φ12+ φ13+, agr- rsbU- tcaR-, r- m- | Iordanescu and Surdeanu, 1976 |
| 8325-4; RN0450 | NRS135 | RN1 derivative; UV mutagenesis; φ11- φ12- φ13-, agr+, rsbU-, tcaR- | Novick, 1967 |
| RN6390 | n.a. | RN1 derivative; UV mutagenesis, φ6390 lysogenized and Tn554 erm insertion; φ11- φ12- φ13-, agr+ rsbU- tcaR- | Peng et al., 1988 |
| RN4220 | NCBI 561307, NRS144, DSM 26309 | RN1 derivative; UV and MNNG mutagenesis; φ11- φ12- φ13-, agr-, rsbU-, tcaR-, r- m- | Kreiswirth et al., 1983 |
| S. carnosus | |||
| TM300 | NCBI 396513 | Meat starter culture bacterium, apathogenic | Rosenstein et al., 2009 |
| P. aeruginosa | |||
| PAO-1 | ATCC 15692, NCBI 208964, DSM 22644 | MDSC-inducing opportunistic pathogen, persists in cystic fibrosis | Stover et al., 2000 |
Strain IDs were taken from American Type Culture Collection (ATCC), National Collection of Type Cultures (NCTC) and from DSMZ, German Collection of Microorganisms and Cell Cultures. JE, NE and NRS numbers correspond to IDs from the Network on Antimicrobial Resistance in Staphylococcus aureus (NARSA) program. Described are relevant genotypic features referring to determination or to regulation of virulence within the scope of this study.
FPR3757 and LAC are different isolates from the same PFGE-type (USA300-0114) of S. aureus. JE2 derivative strains display an insertion of the mariner-based bursa aurealis transposon in the respective genes. Disrupted genes are marked with a ‘-’; deleted loci are denoted with a ‘Δ’. ACME, arginine catabolic mobile element; agr, accessory gene regulator; CA, community-acquired; fnbA, Fibronectin binding protein A; fnbB, Fibronectin binding protein B; GalNAc, N-acetyl-d-galactosamine; GroP, glycerophosphate; HA, hospital-acquired; hlb, hemolysin beta; MRSA, Methicillin-resistant S. aureus; MSSA, Methicillin-susceptible S. aureus; PFGE, pulsed-field gel electrophoresis; PVL, Panton-Valentine leukocidin; r– m–, restriction-modification mutant; rsbU – regulator of sigma factor SigB; saeRS, S. aureus exoprotein expression regulatory system; secA, Sec system protein translocase subunit A; sek, Staphylococcal enterotoxin k; seq, Staphylococcal enterotoxin q; SigB, RNA polymerase sigma factor B; tcaR, Teicoplanin-resistance associated HTH-type transcriptional regulator; Tn, transposon; selX- Staphylococcal enterotoxin-like X; WTA, wall teichoic acid; φ, Prophage Phi. n.a., not available.