Effect of repeated application of 30-min tibial nerve stimulation (TNS) on the micturition reflex. A, Repeated cystometrogram (CMG) traces showing that the amplitude of micturition contraction was partially reduced after the first and second 30-min TNS, but was completely suppressed after the third 30-min TNS. Infusion rate = 1 mL/min. TNS: 5 Hz, 0.2 ms, 4T = 2.4 V. B, Repeated CMGs showing that the amplitude of micturition contraction was completely suppressed after the first 30-min TNS. Infusion rate = 2 mL/min. TNS: 5 Hz, 0.2 ms, 4T = 4.4 V. C and D, summarized results showing the effects on bladder capacity (C) or contraction amplitude (D) after each application of 30-min tibial nerve stimulation (TNS). TNS: 5 Hz, 0.2 ms, 4T = 2.0–4.8 V. T—threshold intensity for inducing toe twitch. *Indicates significantly (P < 0.05) different from control (one-way ANOVA). N = 7 cats