Figure 2.

Fluorescence-guided surgical removal of peritoneal disseminated HCT-116 tumors after GFP labeling with OBP-401. Noncolored HCT-116 human colon cancer cells were injected into the abdominal space of nude mice. Ten days later, 1 × 10⁸ PFU of OBP-401 were i.p. injected. (A) Disseminated nodules were efficiently labeled and noninvasively visualized by GFP expression 5 days after virus administration. (B) Under general anesthesia, laparotomy was performed to remove intra-abdominal disease under GFP-guided navigation. (C) Disseminated nodules visualized by GFP-guided navigation were removed. (Scale bars: A–C, 10 mm.) (D) Frozen section of resected HCT-116 disseminated nodules with fluorescence detection. (Scale bar, 500 µm.) (E) H&E section of HCT-116 disseminated nodules shown in D. The box outlines a region of D and E analyzed in F. (Scale bar, 500 µm.) (F) Detail of the boxed region of D and E. (Scale bar, 50 µm.)[58]