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. 2018 Sep 13;9:2044. doi: 10.3389/fimmu.2018.02044

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Copyright © 2018 Benet, Marthi, Ke, Wu, Turner, Gabayre, Ivanitskiy, Sethi and Grigorova.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Intrinsic production of CCL3 by B cells is required for their control in GCs in mixed bone marrow chimeras. (A) Experimental outline and generation of mixed 50:50 WT/WT (Chimeras I) and WT/CCL3-KO (Chimeras II) BMChs (left) and example of flow cytometry analysis of total CD19⁺ cells and GC cells from draining LNs of BMChs on day 10 p.i. with OVA in CFA (right). (B) Flow cytometry analysis of draining LNs at 0 and 10 d.p.i. Fractions of CD45.1 or CD45.2 GC B cells relative to their respective CD19⁺ B cell population. Data represents four independent experiments. Bars represent means and each symbol represents an individual mouse. *P < 0.05, ANOVA with Dunnet post-analysis.