Figure 6.

The gut microbiota affects intestinal permeability and promotes the natural expression of MUC2 protein and tight junction genes in the CLP-induced Sepsis Model. (A) The intestinal of permeability of different treatment mice (n = 3). The results are shown as means ± SEM, ^*p < 0.05. (B) Tissue sections were prepared from the colons of none-treat WT and Gpr109a^−/– mice, and microbiota-transferred WT and Gpr109a^−/– mice which undergo the CLP surgery (n = 3) and observed by fluorescence microscopy. The nucleus was stained with DAPI (blue) and MUC2 was stained green. Magnification shown is 10×. The scale bar represents 500 μm. (C–H) Representative expression levels of claudin-1 (cldn1), claudin-2 (cldn2), claudin-3 (cldn3), ZO-1, ZO-2, and occludin (ocln) in colon tissue homogenates from different treatment mice (n = 3). The results shown are means ± SEM, ^*p < 0.05.