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. 2018 Sep 13;8:310. doi: 10.3389/fcimb.2018.00310

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Copyright © 2018 Mayer, Muras, Romero, López, Tomás and Otero.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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QQ activity against C6-HSL, C12, and OHC12-HSL (10 μM) in cell extracts of A. baumannii ATCC17978 obtained from 24 h cultures in LB. The purified Aii20J lactonase (20 μg/mL) was used as positive control. The remaining AHL concentration after 3 h of exposure to cell extracts was quantified by HPLC-MS from reaction mixtures aliquots with (black bars) or without (white bars) acidification to reverse the lactonolysis. PBS was used as negative control. Values reported are normalized to the percentage of AHL retrieved from PBS reaction mixtures incubated the same way.