Table 1.
Bacterial strains, plasmids, and primers used in this study.
| Strain or plasmid | Description | Source or references |
|---|---|---|
| STRAINS | ||
| Acinetobacter baumannii | ||
| ATCC17978 | ATCCa | |
| Ab1 (ROC013) | A. baumannii clinical isolate (respiratory). TM: ST2 | INIBICb |
| Ab2 (COR005) | A. baumannii clinical isolate (ulcer). TM: ST186 | INIBICb |
| Ab3 (PON002) | A. baumannii clinical isolate (respiratory). TM: ST52 | INIBICb |
| Ab4 (VAL001) | A. baumannii clinical isolate (respiratory). TM: ST169 | INIBICb |
| Ab5 (DOM009) | A. baumannii clinical isolate (respiratory). TM: ST80 | INIBICb |
| Ab6 (HMV001) | A. baumannii clinical isolate (exudate). TM: ST181 | INIBICb |
| Ab7 (HUI001) | A. baumannii clinical isolate (blood). TM: ST79 | INIBICb |
| Acinetobacter nosocomialis | ||
| M2 | Niu et al., 2008 | |
| Chromobacterium violaceum | ||
| CV026 | AHL biosensor, Kmr | McClean et al., 1997 |
| VIR07 | AHL biosensor, Kmr | Morohoshi et al., 2008 |
| Escherichia coli | ||
| BL21(DE3)plysS | F– ompT hsdSB (rB-, mB-) dcm gal λ(DE3) pLysS Cmr | Promega |
| XL1blue | recA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1 lac [F′ proAB lacIq ZΔM15 Tn10 (Tetr)] | Agilent |
| PLASMIDS | ||
| pET28c(+) | Cloning vector, Kmr | Novagen |
| pET28c(+)-aidA | pET28c(+) containing aidA gene from A. baumannii ATCC17978 | This study |
| pET28c(+)-aii20J | pET28c(+) containing aii20J gene from Tenacibaculum sp. 20J | Mayer et al., 2015 |
| pET28c(+)-A1S_0383 | pET28c(+) containing a1s_0383 gene from A. baumannii ATCC17978 | This study |
| pET28c(+)-A1S_1876 | pET28c(+) containing a1s_1876 gene from A. baumannii ATCC17978 | This study |
| pET28c(+)-A1S_2662 | pET28c(+) containing a1s_2662 gene from A. baumannii ATCC17978 | This study |
| Primers | Sequence (5′-3′) | Use in this work |
| abaI_Fwd | TGTGCCAGACTACTACCCAC | qRT-PCR |
| abaI_Rev | TGCTAGAGGAAGGCGGATTT | qRT-PCR |
| abaR_Fwd | TTGGTCGAGTCAATCTGCAA | qRT-PCR (Eijkelkamp et al., 2013) |
| abaR_Rev | CTCGGGTCCCAATAAAATCA | qRT-PCR (Eijkelkamp et al., 2013) |
| csuD_Fwd | AGTCACAACATCGGTCCCAT | qRT-PCR |
| csuD_Rev | AAGTTCGGTGCGTCCTTCTA | qRT-PCR |
| rpoB_Fwd | GTGCTGACTTGACGCGTGAT | qRT-PCR (Park and Ko, 2015) |
| rpoB_Rev | AGCGTTCAGAAGAGAAGAACAAGTT | qRT-PCR (Park and Ko, 2015) |
| A1S_0383_Fwd | ACCAGGTCACGTCATGTTCT | qRT-PCR |
| A1S_0383_Rev | TGGTACTCATTGGCCCATGT | qRT-PCR |
| A1S_1708_Fwd | ATTGAAGCGCGTTACACACC | qRT-PCR |
| A1S_1708_Rev | ATAGTGTTGTCAGGCAGGCT | qRT-PCR |
| A1S_1876_Fwd | GCAGTCATATGGTCCGCATG | qRT-PCR |
| A1S_1876_Rev | TTAGCAACCCGTCAATGTGC | qRT-PCR |
| A1S_2194_Fwd | TCCCTGGCATTACTCATCCC | qRT-PCR |
| A1S_2194_Rev | TTCAAATAGTCGCCCGCATC | qRT-PCR |
| A1S_2662_Fwd | TCTGCTTCACGTTCATGAGC | qRT-PCR |
| A1S_2662_Rev | CTGCGAGTTGTTTTGGTCCA | qRT-PCR |
| A1S_2864_Fwd | GCCACTGAATACAATGCTGC | qRT-PCR |
| A1S_2864_Rev | TCGCAATACCACAATGTCCG | qRT-PCR |
| aidA_Fwd | TCGCTGCACGTTTTGTACTC | qRT-PCR |
| aidA_Rev | CCATCGGCGTAGTGCTTAAT | qRT-PCR |
| 0383Fwd | GATTAACCATGGTACTGCAAGTCAAAATTGTTCCAG (NcoI)c | Cloning |
| 0383Rev | GCTATGAATTCAAACCCGCTTTACCTGCGACAAACG (EcoRI)c | Cloning |
| 1876Fwd | GATTAACCATGGTACAACAACCTCTAGTAAAAGA(NcoI)c | Cloning |
| 1876Rev | GCTATGAATTCAAAAAGTAATTAAATGGGATT (EcoRI)c | Cloning |
| 2662Fwd | GATTAACCATGGTAAAAAAACTATTTGTAGCCTTAGG (NcoI)c | Cloning |
| 2662Rev | GCTATGAATTCAATTTATCAAGACTGTTATTA (EcoRI)c | Cloning |
| aidAFwd | GATTAACCATGGTA.GGTAAAAGTCTAAATAATGT | Cloning |
| aidARev | GCTATGAATTCAACTTGACTGGAACGATGCGTTTA | Cloning |
| T7Fwd | TAATACGACTCACTATAGGGGAA | Universal primer |
| T7Rev | GCTAGTTATTGCTCAGCGG | Universal primer |
| luxI PF | GGTTGGGAGTTGAACTGTCC | abaI amplification (Bitrian et al., 2012) |
| luxI PR | GGTTGGGAGTTGAACTGTCC | abaI amplification (Bitrian et al., 2012) |
| luxR PF | TCGGATTTGATTATTGCGCTTATG | abaI amplification (Bitrian et al., 2012) |
| luxR PR | ACAGCTCGAATAGCTGCTG | abaI amplification (Bitrian et al., 2012) |
a
American Type Culture Collection.
b
Instituto de Investigación Biomédica (A Coruña).
c
Restriction sites for indicated enzymes are underlined.