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(A) Bioinformatics analysis predicted miR-206-binding sites of miR-206 in TM4SF1 3′-UTR. (B) Relative luciferase activity of wild-type or mutant TM4SF1 in HCT116 and Caco-2 cells. (C) Relative miR-206 expression in HCT116 cells following transfection with miR-206. Relative TM4SF1 mRNA (D) and protein (E) expression levels in HCT116. *P<0.05, **P<0.01, and ***P<0.001 compared with the miR-NC. The data are presented as mean ± standard error.
