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(a) Ellipsoidal volume of interest extracted around the center of the lacuna, segmented to remove the bone matrix, (b) skeletonization of the LCN in the vicinity of the lacuna. This procedure yields the number of primary canaliculi directly connected to the lacuna, Ca.Pr, and the length of the first branching distance, Ca.Db. The white scale bar in (a) represents 1 μm.
