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. 2018 Jul 30;8(5):772–783. doi: 10.1016/j.apsb.2018.07.006

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© 2018 Chinese Pharmaceutical Association and Institute of Materia Medica, Chinese Academy of Medical Sciences. Production and hosting by Elsevier B.V.

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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SPR analysis for the binding of compounds IMB-84 and IMB-87 to proteins L12 and L10 and their effect on L12–L10 protein interaction. (A) and (B) Compounds IMB-84 and IMB-87 bind to L10 protein. Solutions with series concentrations of IMB-84 (A) or IMB-87 (B) were injected into the L10-coated sensor chip chamber. The change of response units is shown. (C) and (D) Compounds IMB-84 and IMB-87 bind to L12 protein. Solutions with series concentrations of IMB-84 (C) or IMB-87 (D) were injected into the L12-coated sensor chip chamber. The change of response units is shown. (E) Binding of the GST and GST-L12 to L10 protein. Solutions of GST or GST-L12 were injected into the L10-coated sensor chip chamber. The change of response units is shown. (F) Compounds IMB-84 and IMB-87 block L12–L10 interaction. IMB-84 or IMB-87 (25 μmol/L) was injected into the L12-coated sensor chip chamber. Then purified L10 protein was injected. As a control, HBS-P⁺ was injected at first and followed by the injection of L10. The change of response units was measured.