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In vitro inhibition of GTPase activity and protein translation by IMB-84 and IMB-87. (A) and (B) Compounds IMB-84 and IMB-87 reduce ribosome GTPase activity. The GTPase activity was assessed by measuring free Pi from GTP using malachite green solution. (C) and (D) Inhibition of protein translation by IMB-84 and IMB-87. Inhibition was determined using cell-free translation system containing E. coli or rabbit reticulocyte, and luciferase was used as a reporter.
