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. Author manuscript; available in PMC: 2019 Sep 1.
Published in final edited form as: Obesity (Silver Spring). 2018 Sep;26(9):1439–1447. doi: 10.1002/oby.22254

Figure 3. OSMRFKO mice exhibit a modest blunting of insulin-induced Akt phosphorylation.

Figure 3

A) Body weights, B) 4-hour fasting blood glucose, and C) protein expression levels of Akt, phosphoAkt, and phosphoERK in eWAT from chow-fed OSMRfl/fl and OSMRFKO (KO) mice. Body weights and blood glucose levels were not significantly different between genotypes. 100 ug protein was subjected to Western blot analysis. KO mice exhibited a slight diminution in Akt phosphorylation (S473) in eWAT. Densitometry analysis of D) pAkt/Akt ratio was conducted using Image Studio software. For vehicle injections, n=3 per genotype and for insulin injections, n=4 per genotype. Data are shown as mean ± SEM. AU, arbitrary units.