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. Author manuscript; available in PMC: 2019 Sep 1.
Published in final edited form as: Obesity (Silver Spring). 2018 Sep;26(9):1439–1447. doi: 10.1002/oby.22254

Figure 5. Adipokine gene expression in 3T3-L1 adipocytes in response to OSM treatment and/or ERK inhibition.

Figure 5

Fully differentiated 3T3-L1 adipocytes were treated with 1 nM or OSM or vehicle for 6 hours in the presence/absence of the ERK inhibitor U0126. Gene expression levels of A) Lcn2, B) Igfbp6, C) Icam1, Crp, and FetA were assessed, but Crp and FetA were not detected. Total RNA was purified from cells and analyzed by real-time PCR. Ubiquitin b (Ubb) was used as an endogenous control.