Figure 4.

Intestinal PMK-1/p38 MAPK mediates the oxidative stress-induced decrease of DCVs in motor axons. A, Left, Representative images of the distribution of the proneuropeptide INS-22::YFP driven by the unc-17 promoter in cholinergic motor neurons in young adult wild-type, pmk-1 mutants, or pmk-1 mutants expressing pmk-1 cDNA under the intestine-specific promoter ges-1 in the presence or absence of arsenite (As). Right, Quantification of the puncta fluorescence of INS-22::YFP of the indicated strains in the absence or presence of arsenite. B, Fold change of INS-22::YFP punctal fluorescence following arsenite treatment of the indicated strains. Numbers of animals tested are indicated in white. Scale bar, 10 μm. Error bars indicate ± SEM. Student's t test, **p < 0.01, ***p < 0.001.