Figure 4.

Lack of 3,5-DHPG modulation of glycinergic eIPSCs. A, B, Amplitude of individual (top) and averaged glycinergic eIPSCs (bottom) remained relatively constant during 3,5-DHPG application compared with control in both WT and Fmr1 KO neurons. C, Normalized glycinergic eIPSC amplitude is not affected by 3,5-DHPG in WT neurons (n = 10). D, In Fmr1 KO neurons, normalized glycinergic eIPSC amplitude is not affected by 3,5-DHPG, although eIPSC amplitude was briefly suppressed within the first minute of 3,5-DHPG application and showed some facilitation during wash (n = 5). No significant differences between drug conditions or genotypes were observed for mean glycinergic eIPSC amplitude (E), normalized amplitude (F), PPR (G), or decay time constant (tau) (H) (WT: n = 10; Fmr1 KO: n = 5).