Fig. 5.

Phenotypic characterization of SAPK1-OE and SAPK2-OE plants at germination and post germination stage. (a) Seed germination analyses: the germination of wild-type (WT), SAPK1-OE and SAPK2-OE plants’ seeds on day 6 after stratification on medium supplemented with different concentrations of NaCl. (b) The germination rates of WT, SAPK1-OE and SAPK2-OE plants’ seeds on 175 mM NaCl medium over 6 days. All experiments were performed three times each, evaluating more than 60 seeds. (c) Early seedling development analyses: WT, SAPK1-OE and SAPK2-OE plants’ seeds were geminated in MS medium, and after 3 days all were transferred to 175 mM NaCl-containing medium in a vertical manner. The pictures of representative seedlings were taken 7 days after transfer. (d) Shoot and (e) Root elongation analyses corresponding to c. Error bars indicate the SD (n = 3). * indicate statistically significant differences between overexpression lines and wild type plants (P < 0.05). ** indicate statistically significant differences between overexpression lines and wild type plants (P < 0.01)