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. 2018 Sep 20;15:273. doi: 10.1186/s12974-018-1315-1

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© The Author(s). 2018

Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Immunofluorescence analysis and Western blotting of Iba-1 expression in the injured cortex and ipsilateral hippocampus. a Number of Iba-1-positive cells in the injured cortex and ipsilateral hippocampus. Data in the bar graphs represent mean ± SD. ****P < 0.001. At least 10 randomly selected microscopic fields were used for counting (magnification, × 630). b, c Western blotting of Iba-1 from the injured cortex and ipsilateral hippocampus. Data in the bar graphs represent mean ± SD. β-Actin was used as the load control. **P < 0.01; ****P < 0.001