| Time | % Eluent A | % Eluent B | Flow (mL/min) |
|---|---|---|---|
| 0 | 65 | 35 | 1.0 |
| 10 | 65 | 35 | 1.0 |
| 12 | 90 | 10 | 2.0 |
| 18 | 90 | 10 | 2.0 |
| 20 | 65 | 35 | 1.0 |
| 25 | 65 | 35 | 1.0 |
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