Fig. 1.

Confluent and adherent primary mouse bone marrow endothelial cells (BMECs) were treated for 24 h with the conditioned medium (CM) from vehicle or ISO-treated BMSCs prior to addition of GFP-positive (+ ) MDA-MB-231 (A) or GFP +  4T1 (B) cells. After 3.5 h, wells were washed in PBS and the relative adhesion of cancer cells to the endothelial monolayer was quantified by fluorescence-labeled cell count assay. Results are graphed as fold change relative to the CM of PBS-treated BMECs (n = 4 independent experiments, * = p < 0.05, *** = p < 0.001). A and B, right panel: Representative images of tumor cells attached to BMEC monolayers after treatment (blue: DAPI nuclear staining, green: GFP +  tumor cells). Scale bar  =  100 µm. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)