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. 2018 Sep 13;19:412–428. doi: 10.1016/j.redox.2018.09.009

Fig. 2.

Fig. 2

Expression of Mstn in human cancer and non-tumorigenic HEK293 cells, the Mstn knockdown inhibited growth of cancer cells. (A) Immunoblot analyses of Mstn expression in human cancer (Huh7, HeLa, HepG2, and A549) and non-tumorigenic HEK293 cells. The antibodies used are indicated on the left. Each blot is representative of three independent experiments. (B) Semi-quantitative densitometric analyses of the full length Mstn and the mature Mstn shown in (A) using Image J software. The protein content was normalized against the corresponding β-actin level. Data represent the mean ± standard error of the mean based on three independent experiments, which were analyzed by one-way ANOVA. ***P < 0.0001. (C) The mRNA levels of Mstn in shRNAs stably expressing cells were detected by Q-PCR. Values were normalized relative to the β-Actin mRNA levels. Data represent the mean ± standard error of the mean based on three independent experiments. *P < 0.05, **P < 0.01, one-way ANOVA. (D) Proliferation of control-shRNA or Mstn-shRNAs stably expressing Huh7 cells was determined using the CCK8 assay. Data represent the mean ± standard error of the mean based on three independent experiments, which were analyzed by one-way ANOVA. ***P < 0.0001. (E) Proliferation of control-shRNA or Mstn-shRNAs stably expressing HeLa cells was determined using the CCK8 assay. Data represent the mean ± standard error of the mean based on three independent experiments, which were analyzed by one-way ANOVA. *P < 0.05, **P < 0.01, ***P < 0.0001. (F) Proliferation of control-shRNA or Mstn-shRNAs stably expressing HepG2 cells was determined using the CCK8 assay. Data represent the mean ± standard error of the mean based on three independent experiments, which were analyzed by one-way ANOVA. ***P < 0.0001. (G) Proliferation of control-shRNA or Mstn-shRNAs stably expressing HEK293 cells was determined using the CCK8 assay. Data represent the mean ± standard error of the mean based on three independent experiments, which were analyzed by one-way ANOVA. *P < 0.05. (H) Proliferation of control-shRNA or Mstn-shRNAs stably expressing A549 cells was determined using the CCK8 assay. Data represent the mean ± standard error of the mean based on three independent experiments, which were analyzed by one-way ANOVA. ***P < 0.0001.