Fig. 5.

Mstn KO inhibited growth of tumor xenograft in nude mice. (A) Representative mice bearing tumors after 34 days post inoculation. Black arrowheads, inoculation sites. (B) Cells were injected into nude mice and four representative xenograft tumors in each group are shown. Scale bar, 1 cm. (C) Tumor sizes were measured on the days indicated and the tumor volumes were calculated. Data represent the mean ± standard error of the mean (n = 8 per group). ^*P < 0.05, ^**P < 0.01, ^***P < 0.0001, one-way ANOVA. (D) Tumor weights are shown in the scatter plot. Data represent the mean ± standard error of the mean (n = 8 per group). ^***P < 0.0001, unpaired two-tailed t-test. (E) Representative images of fluorescent TUNEL and DAPI staining of xenograft tumor sections. The boxed region in the image is enlarged below. Scale bar, 50 µm. The percentages of apoptotic cells in tumor xenografts were quantified and normalized versus the number of nuclei. At least 300 cells from each section were counted. Data represent the mean ± standard error of the mean. ^***P < 0.0001, unpaired two-tailed t-test. (F) HeLa/Mstn KO cells were injected into nude mice and PBS or Mstn were injected intratumorally. Four representative xenograft tumors in each group with the treatment of PBS or Mstn were shown. Scale bar, 1 cm. (G) Tumor sizes were measured on the days indicated and the tumor volumes were calculated. Data represent the mean ± standard error of the mean (n = 8 per group). ^*P < 0.05, ^***P < 0.0001, one-way ANOVA. (H) Tumor weights are shown in the scatter plot. Data represent the mean ± standard error of the mean (n = 8 per group). ^*P < 0.05, unpaired two-tailed t-test.