Table 1.
Sequences of bovine oligonucleotide primers used for real-time reverse transcription PCR
| Gene symbol | Accession numbera | Primer sequence (5′ →3′)b |
|---|---|---|
| SLC2A4 d | NM_174604.1 | F: GGGACTGGTACCCATGTACG |
| R: AGGAGGAGTGGCCATAAGGT | ||
| ACAT1 d | NM_001046075.1 | F: TCAACGGAGGAGCTGTTTCT |
| R: TGCAAATACTGGCAAGACCA | ||
| HMGCoAS d | NM_001045883.1 | F: AGAACGTCTGCCCTCTTTCA |
| F: TACAAGGCTGCTGTGTCCAG | ||
| SREBP1c d | NM_001113302.1 | F: CTACATCCGCTTCCTTCAGC |
| R: TTCAGCGATTTGCTTTTGTG | ||
| FASN d | NM_001012669.1 | F: TCATCCCCCTGATGAAGAAG |
| R: AACTCCACAGGTGGGAACAG | ||
| ACLY d | NM_001037457.1 | F: CAAGAAGGCAGACCAGAAGG |
| R: CTGGGCGGTACAGCTTAGAG | ||
| ELOVL6 d | NM_001102155.1 | F: GGAAAGCAACGAAAGCTGAC |
| R: TGGGTTGTGTGTTTGCTCAT | ||
| SCD d | NM_173959.4 | F: CGACGTGGCTTTTTCTTCTC |
| R: GATACCATGGCACGAGTGTG | ||
| ACACA d | NM_174224.2 | F: TGTCCGAAACGTCGATTTTTG |
| R: ACGACCTGGTTGCTGTGATAGA | ||
| HMGCoAR d | NM_001105613.1 | F: TGAGGGAGAACATTGCTCGT |
| R: ACATGATTTCGAGCTGACGC | ||
| RFLPO c | NM_001012682 | F: CAACCCTGAAGTGCTTGACAT |
| R: AGGCAGATGGATCAGCCA | ||
| UTX2 c | NM_001037471 | F: TGTGGCCCTTGGATATGGTT |
| R: GGTTGTCGCTGAGCTCTGTG | ||
| TPB c | NM_001075742 | F: CCTAAAGACCATTGCACTTCG |
| R: CTTCACTCTTGGCTCCTGTG | ||
| TOP2B c | XM_001254709 | F: CCGATGATGATGACGACAAT |
| R: TGCTATGGGAGATGCTTTGA | ||
| B-actin c | U39357 | F: CTCACGGAGCGTGGCTACA |
| R: GCCATCTCCTGCTCGAAGTC |
aAccession number in the National Centre for Biotechnology Information database (www.ncbi.nlm.nih/gov/gene)
bF forward primer, R reverse primer
cGenes used for as reference for qPCR normalisation; RFLPO, Ribosomal protein large PO; UTX2, Ubiquitously expressed transcript; TPB, TATA box-binding protein; Topoisomerase II-beta TOP2B, β-actin
dTarget genes; SLC2A4 solute carrier family 2 member 4, ACAT1 Acetyl-CoA Acetyltransferase 1, HMGCoAS Hydroxymethylglutaryl CoA synthase, SREBP1c Sterol regulatory element-binding protein, FASN Fatty acid synthase, ACLY Acetyl citrate lyase, ELOVL6 Fatty acid elongase 6, SCD Stearoyl-CoA desaturase-1, ACACA Acetyl-CoA carboxylase, HMGCoAR Hydroxymethylglutaryl CoA reductase