Appendix.
Multilocus microsatellite profiles of isolates used in this study using primers developed for Ceratocystis sp. (Part 1)
| Species | CMW Culture number | Personal Collection number | Host | ITS haplotype | Country/Locality | Date collected |
Fourie et al. 2016 |
||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| AF2 | AF3 | AF4 | AF5 | AF6 | AF7 | AF8 | AF9 | AF11 | |||||||
| Latin American Clade (LAC) | |||||||||||||||
| C. fimbriata | 46734 | P15-31 | Ipomoea batatas | - | USA, Hawai’i Island | 2015 | 198 | 215 | 246 | 242 | 285 | 331 | 349 | 415 | 460 |
| Ceratocystis sp. | 48499 | C1817 | Xanthosoma sp. | - | Cuba | 2001 | 206 | 203 | 254 | 262 | 300 | 325 | 334 | 415 | 432 |
| Ceratocystis sp. | 48506 | C1780 | Xanthosoma sagittifolium | - | Costa Rica | 2001 | 209 | 203 | 257 | 262 | 300 | 325 | 334 | 415 | 432 |
| Ceratocystis sp. | 48500 | C1717 | Syngonium sp. | - | USA, Hawai’i | - | 192 | 206 | 252 | 258 | 294 | 325 | 334 | 415 | 432 |
| Ceratocystis sp. | 48508 | C1809 | Syngonium podophyllum | - | USA, Florida | 2001 | 192 | 206 | 252 | 258 | 294 | 325 | 334 | 415 | 432 |
| Ceratocystis sp. | 50456 | P16-1 | Syngonium podophyllum | - | USA, Hawai’i, Hilo, South Hilo District | 2016 | 192 | 206 | 252 | 258 | 294 | 325 | 334 | 415 | 432 |
| Ceratocystis sp. | 50457 | P16-2 | Syngonium podophyllum | - | USA, Hawai’i, Hilo, South Hilo District | 2016 | 192 | 206 | 252 | 258 | 294 | 325 | 334 | 415 | 432 |
| C. lukuohia | 44102 | P14-1-1 | Metrosideros polymorpha | A | USA, Hawai’i, Leilani Estates, Puna District | 2014 Feb 20 | 195 | 215 | 249 | 262 | NR | 316 | 334 | 412 | 432 |
| C. lukuohia | 44105 | P14-12 | Metrosideros polymorpha | A | USA, Hawai’i, Hawaiian Acres, Puna District | 2014 Jul 31 | 195 | 215 | 249 | 262 | NR | 316 | 334 | 412 | 432 |
| C. lukuohia | 44106 | P14-13 | Metrosideros polymorpha | A | USA, Hawai’i, Hawaiian Paradise Park, Puna District | 2014 Jul 8 | 195 | 215 | 249 | 262 | NR | 316 | 334 | 412 | 432 |
| C. lukuohia | 44107 | P14-15 | Metrosideros polymorpha | A | USA, Hawai’i, Hawaiian Acres, Puna District | 2014 Jul 31 | 195 | 215 | 249 | 262 | NR | 316 | 334 | 412 | 432 |
| C. lukuohia | 44103 | P14-6 | Metrosideros polymorpha | A | USA, Hawai’i, Orchidlands Estates, Puna District | 2014 Jun 11 | 195 | 215 | 249 | 262 | NR | 316 | 334 | 412 | 432 |
| C. lukuohia | 47857 | P15-11 | Metrosideros polymorpha | C | USA, Hawai’i, Keau’ohana Forest Reserve, Puna District | 2015 Jan 6 | 195 | 215 | 249 | 262 | NR | 316 | 334 | 412 | 432 |
| C. lukuohia | 47139 | P15-17 | Metrosideros polymorpha | Mix A/C | USA, Hawai’i, Mālama Kî Forest Reserve, Puna District | 2015 Feb 2 | 195 | 215 | 249 | 262 | NR | 316 | 334 | 412 | 432 |
| C. lukuohia | 46743 | P15-27 | Metrosideros polymorpha | Mix A/C | USA, Hawai’i, Kopua Farm Lots, Puna District | 2015 Mar 12 | 195 | 215 | 249 | 262 | NR | 316 | 334 | 412 | 432 |
| C. lukuohia | 46741 | P15-64 | Metrosideros polymorpha | C | USA, Hawai’i, Nānāwale Estates, Puna District | 2015 Jun 3 | 195 | 215 | 249 | 262 | NR | 316 | 334 | 412 | 432 |
| C. lukuohia | 47152 | P15-65 | Metrosideros polymorpha | Mix A/C | USA, Hawai’i, Waiākea Forest Reserve, South Hilo District | 2015 May 27 | 195 | 215 | 249 | 262 | NR | 316 | 334 | 412 | 432 |
| C. lukuohia | 47153 | P15-67 | Metrosideros polymorpha | Mix A/C | USA, Hawai’i, Keau’ohana Forest Reserve, Puna District | 2015 Jun 9 | 195 | 215 | 249 | 262 | – | 316 | 334 | 412 | 432 |
| C. lukuohia | 47154 | P15-68 | Metrosideros polymorpha | Mix A/C | USA, Hawai’i, Volcano, Puna District | 2015 Jul 15 | 195 | 215 | 249 | 262 | – | 316 | 334 | 412 | 432 |
| C. lukuohia | 46702 | P14-1-3 | Metrosideros polymorpha | A | USA, Hawai’i, Leilani Estates, Puna District | 2014 Feb 20 | 195 | 215 | 249 | 262 | – | 316 | 334 | 412 | – |
| C. lukuohia | 46706 | P14-4-3 | Metrosideros polymorpha | USA, Hawai’i, Orchidlands Estates, Puna District | 2014 Jun 11 | 195 | 215 | 249 | 262 | – | 316 | 334 | 412 | – | |
| C. lukuohia | 46707 | P14-4-4 | Metrosideros polymorpha | USA, Hawai’i, Orchidlands Estates, Puna District | 2014 Jun 11 | 195 | 215 | 249 | 262 | – | 316 | 334 | 412 | – | |
| C. lukuohia | 46711 | P14-7 | Metrosideros polymorpha | A | USA, Hawai’i, Orchidlands Estates, Puna District | 2014 Jun 11 | 195 | 215 | 249 | 262 | – | 316 | 334 | 412 | – |
| C. lukuohia | 47856 | P15-10 | Metrosideros polymorpha | Mix A/C | USA, Hawai’i, Keau’ohana Forest Reserve, Puna District | 2015 Jan 26 | 195 | 215 | 249 | 262 | – | 316 | 334 | 412 | – |
| C. lukuohia | 46725 | P15-15 | Metrosideros polymorpha | Mix A/C | USA, Hawai’i, Nānāwale Estates, Puna District | 2015 Jan 29 | 195 | 215 | 249 | 262 | – | 316 | 334 | 412 | – |
| C. lukuohia | 47140 | P15-18 | Metrosideros polymorpha | A | USA, Hawai’i, Mālama Kî Forest Reserve, Puna District | 2015 Feb 2 | 195 | 215 | 249 | 262 | – | 316 | 334 | 412 | – |
| C. lukuohia | 47858 | P15-20 | Metrosideros polymorpha | Mix A/C | USA, Hawai’i, Mālama Kî Forest Reserve, Puna District | 2015 Feb 2 | 195 | 215 | 249 | 262 | – | 316 | 334 | 412 | – |
| C. lukuohia | 47854 | P15-6 | Metrosideros polymorpha | Mix A/C | USA, Hawai’i, Leilani Estates, Puna District | 2015 Jan 13 | 195 | 215 | 249 | 262 | – | 316 | 334 | 412 | – |
| C. lukuohia | 46722 | P15-7 | Metrosideros polymorpha | Mix A/C | USA, Hawai’i, Leilani Estates, Puna District | 2015 Jan 15 | 195 | 215 | 249 | 262 | – | 316 | 334 | 412 | – |
| C. lukuohia | 47855 | P15-8 | Metrosideros polymorpha | A | USA, Hawai’i, Leilani Estates, Puna District | 2015 Jan 15 | 195 | 215 | 249 | 262 | – | 316 | 334 | 412 | – |
| C. lukuohia | 46723 | P15-9 | Metrosideros polymorpha | USA, Hawai’i, Leilani Estates, Puna District | 2015 Jan 15 | 195 | 215 | 249 | 262 | – | 316 | 334 | 412 | – | |
| C. lukuohia | 46710 | P14-6-1 | Metrosideros polymorpha | A | USA, Hawai’i, Orchidlands Estates, Puna District | 2014 Jun 11 | 195 | 215 | 249 | 262 | – | 316 | 334 | 412 | 432 |
| C. lukuohia | 46714 | P14-11 | Metrosideros polymorpha | Mix A/C | USA, Hawai’i, Hawaiian Acres, Puna District | 2014 Jul 31 | 195 | 215 | 249 | 262 | – | 316 | 334 | 412 | 432 |
| C. lukuohia | 46718 | P14-16 | Metrosideros polymorpha | Mix A/C | USA, Hawai’i, Hawaiian Acres, Puna District | 2014 Jul 31 | 195 | 215 | 249 | 262 | – | 316 | 334 | 412 | 432 |
| C. lukuohia | 46703 | P14-2 | Metrosideros polymorpha | Mix A/C | USA, Hawai’i, Orchidlands Estates, Puna District | 2014 Jun 11 | 195 | 215 | 249 | 262 | – | 316 | 334 | 412 | 432 |
| C. lukuohia | 46704 | P14-2-1 | Metrosideros polymorpha | Mix A/C | USA, Hawai’i, Orchidlands Estates, Puna District | 2014 Jun 11 | 195 | 215 | 249 | 262 | – | 316 | 334 | 412 | 432 |
| C. lukuohia | 46705 | P14-3 | Metrosideros polymorpha | Mix A/C | USA, Hawai’i, Orchidlands Estates, Puna District | 2014 Jun 11 | 195 | 215 | 249 | 262 | – | 316 | 334 | 412 | 432 |
| C. lukuohia | 46712 | P14-7-3 | Metrosideros polymorpha | USA, Hawai’i, Orchidlands Estates, Puna District | 2014 Jun 11 | 195 | 215 | 249 | 262 | – | 316 | 334 | 412 | 432 | |
| C. lukuohia | 46713 | P14-8 | Metrosideros polymorpha | A | USA, Hawai’i, Orchidlands Estates, Puna District | 2014 Jun 11 | 195 | 215 | 249 | 262 | – | 316 | 334 | 412 | 432 |
| C. lukuohia | 46719 | P15-1 | Metrosideros polymorpha | Mix A/C | USA, Hawai’i, Waiākea Forest Reserve, South Hilo District | 2014 Nov 20 | 195 | 215 | 249 | 262 | – | 316 | 334 | 412 | 432 |
| C. lukuohia | 47138 | P15-13 | Metrosideros polymorpha | A | USA, Hawai’i, Nānāwale Estates, Puna District | 2015 Jan 29 | 195 | 215 | 249 | 262 | – | 316 | 334 | 412 | 432 |
| C. lukuohia | 46724 | P15-14 | Metrosideros polymorpha | A | USA, Hawai’i, Nānāwale Estates, Puna District | 2015 Jan 29 | 195 | 215 | 249 | 262 | – | 316 | 334 | 412 | 432 |
| C. lukuohia | 46726 | P15-16 | Metrosideros polymorpha | Mix A/C | USA, Hawai’i, Nānāwale Estates, Puna District | 2015 Jan 29 | 195 | 215 | 249 | 262 | – | 316 | 334 | 412 | 432 |
| C. lukuohia | 46727 | P15-19 | Metrosideros polymorpha | Mix A/C | USA, Hawai’i, Mālama Kî Forest Reserve, Puna District | 2015 Feb 2 | 195 | 215 | 249 | 262 | – | 316 | 334 | 412 | 432 |
| C. lukuohia | 46720 | P15-2 | Metrosideros polymorpha | Mix A/C | USA, Hawai’i, Waiākea Forest Reserve, South Hilo District | 2014 Nov 20 | 195 | 215 | 249 | 262 | – | 316 | 334 | 412 | 432 |
| C. lukuohia | 46728 | P15-21 | Metrosideros polymorpha | A | USA, Hawai’i, Leilani Estates, Puna District | 2014 Jun 17 | 195 | 215 | 249 | 262 | – | 316 | 334 | 412 | 432 |
| C. lukuohia | 46729 | P15-22 | Metrosideros polymorpha | Mix A/C | USA, Hawai’i, Leilani Estates, Puna District | 2015 Jan 27 | 195 | 215 | 249 | 262 | – | 316 | 334 | 412 | 432 |
| C. lukuohia | 46730 | P15-24 | Metrosideros polymorpha | A | USA, Hawai’i, ‘Opihikao, Puna District | 2015 Mar 3 | 195 | 215 | 249 | 262 | – | 316 | 334 | 412 | 432 |
| C. lukuohia | 46731 | P15-25 | Metrosideros polymorpha | A | USA, Hawai’i, ‘Opihikao, Puna District | 2015 Mar 3 | 195 | 215 | 249 | 262 | – | 316 | 334 | 412 | 432 |
| C. lukuohia | 46732 | P15-28 | Metrosideros polymorpha | Mix A/C | USA, Hawai’i, Hawaiian Acres, Puna District | 2015 Mar 12 | 195 | 215 | 249 | 262 | – | 316 | 334 | 412 | 432 |
| C. lukuohia | 46733 | P15-29 | Metrosideros polymorpha | Mix A/C | USA, Hawai’i, Pu’u Kali’u, Puna District | 2015 Mar 6 | 195 | 215 | 249 | 262 | – | 316 | 334 | 412 | 432 |
| C. lukuohia | 47142 | P15-32 | Metrosideros polymorpha | A | USA, Hawai’i, Pu’u Kali’u, Puna District | 2015 Mar 6 | 195 | 215 | 249 | 262 | – | 316 | 334 | 412 | 432 |
| C. lukuohia | 46735 | P15-34 | Metrosideros polymorpha | Mix A/C | USA, Hawai’i, Hawaiian Paradise Park, Puna District | 2015 Mar 12 | 195 | 215 | 249 | 262 | – | 316 | 334 | 412 | 432 |
| C. lukuohia | 47144 | P15-35 | Metrosideros polymorpha | Mix A/C | USA, Hawai’i, Hawaiian Paradise Park, Puna District | 2015 Mar 12 | 195 | 215 | 249 | 262 | – | 316 | 334 | 412 | 432 |
| C. lukuohia | 47145 | P15-36 | Metrosideros polymorpha | Mix A/C | USA, Hawai’i, Volcano, Puna District | 2015 Mar 3 | 195 | 215 | 249 | 262 | – | 316 | 334 | 412 | 432 |
| C. lukuohia | 47146 | P15-38 | Metrosideros polymorpha | Mix A/C | USA, Hawai’i, Lava Tree, Puna District | 2015 Mar 3 | 195 | 215 | 249 | 262 | – | 316 | 334 | 412 | 432 |
| C. lukuohia | 47147 | P15-39 | Metrosideros polymorpha | Mix A/C | USA, Hawai’i, Lava Tree, Puna District | 2015 Mar 3 | 195 | 215 | 249 | 262 | – | 316 | 334 | 412 | 432 |
| C. lukuohia | 47136 | P15-4 | Metrosideros polymorpha | Mix A/C | USA, Hawai’i, Orchidlands Estates, Puna District | 2014 Dec 12 | 195 | 215 | 249 | 262 | – | 316 | 334 | 412 | 432 |
| C. lukuohia | 46708 | P14-5 | Metrosideros polymorpha | Mix A/C | USA, Hawai’i, Orchidlands Estates, Puna District | 2014 Jun 11 | 195 | 215 | 249 | 262 | – | 316 | 334 | 412 | 432 |
| C. lukuohia | 47137 | P15-5 | Metrosideros polymorpha | Mix A/C | USA, Hawai’i, Orchidlands Estates, Puna District | 2014 Dec 12 | 195 | 215 | 249 | 262 | – | 316 | 334 | 412 | 432 |
| C. lukuohia | 46736 | P15-53 | Metrosideros polymorpha | Mix A/C | USA, Hawai’i, Orchidlands Estates, Puna District | 2015 Mar 31 | 195 | 215 | 249 | 262 | – | 316 | 334 | 412 | 432 |
| C. lukuohia | 47148 | P15-54 | Metrosideros polymorpha | Mix A/C | USA, Hawai’i, Leilani Estates, Puna District | 2015 May 27 | 195 | 215 | 249 | 262 | – | 316 | 334 | 412 | 432 |
| C. lukuohia | 46737 | P15-55 | Metrosideros polymorpha | Mix A/C | USA, Hawai’i, Hilo Watershed Forest Reserve, South Hilo District | 2015 Jun 8 | 195 | 215 | 249 | 262 | – | 316 | 334 | 412 | 432 |
| C. lukuohia | 47150 | P15-61 | Metrosideros polymorpha | Mix A/C | USA, Hawai’i, Hilo Watershed Forest Reserve, South Hilo District | 2015 Jul 1 | 195 | 215 | 249 | 262 | – | 316 | 334 | 412 | 432 |
| C. lukuohia | 46740 | P15-63 | Metrosideros polymorpha | Mix A/C | USA, Hawai’i, ‘Opihikao, Puna District | 2015 May 27 | 195 | 215 | 249 | 262 | – | 316 | 334 | 412 | 432 |
| C. lukuohia | 46742 | P15-66 | Metrosideros polymorpha | A | USA, Hawai’i, Keau’ohana Forest Reserve, Puna District | 2015 Jun 9 | 195 | 215 | 249 | 262 | – | 316 | 334 | 412 | 432 |
| C. platani | 9499 | CF20 | Platanus sp. | - | Italy | - | 203 | 239 | 243 | 262 | NR | 322 | 334 | 418 | 432 |
| C. platani | 1896 | BRENO10 | Platanus sp. | - | Switzerland | - | 203 | 239 | 243 | 262 | NR | 322 | 334 | 418 | 432 |
| C. platani | 2219 | 1012/D0 | Platanus sp. | - | France, Saint-Maurice | 1991 | 203 | 239 | 243 | 262 | NR | 322 | 334 | 418 | 432 |
| C. platani | 23450 | c-1 | Platanus orientalis | - | Greece | 2006 | 203 | 239 | 243 | 262 | NR | 322 | 334 | 418 | 432 |
| C. platani | 14802 | C1317 | Platanus occidentalis | - | USA, North Carolina | 1998 Jul | 201 | 215 | 243 | 262 | – | 322 | 334 | 426 | – |
| C. platani | 9496 | CF34 | Platanus sp. | - | Italy | - | 203 | 239 | 243 | 262 | – | 322 | 334 | 418 | – |
| C. platani | 9000 | CF8 | Platanus sp. | - | Italy | 2002 Feb | 203 | 239 | 243 | 262 | – | 322 | 334 | 418 | – |
| C. platani | 2218 | 1012/D4 | Platanus sp. | - | France, Saint-Maurice | 1991 | 203 | 239 | 243 | 262 | – | 322 | 334 | 418 | – |
| C. platani | 23918 | Platanus sp. | - | Greece | 2006 Jul | 203 | 239 | 243 | 262 | – | 322 | 334 | 418 | – | |
| Asian_Australian Clade (AAC) | |||||||||||||||
| C. huliohia | 47135 | P14-10 | Metrosideros polymorpha | B | USA, Hawai’i, ‘Opihikao, Puna District | 2014 Jul 8 | 212 | 194 | 234 | 226 | NR | 328 | 328 | 401 | 444 |
| C. huliohia | 46716 | P14-14 | Metrosideros polymorpha | B | USA, Hawai’i, Hawaiian Paradise Park, Puna District | 2014 Jul 8 | 212 | 194 | 234 | 226 | NR | 328 | 328 | 401 | 444 |
| C. huliohia | 44104 | P14-9-1 | Metrosideros polymorpha | B | USA, Hawai’i, Hawaiian Paradise Park, Puna District | 2015 Feb 04 | 212 | 194 | 234 | 226 | NR | 328 | 328 | 401 | 444 |
| C. huliohia | 46721 | P15-3 | Metrosideros polymorpha | B | USA, Hawai’i, Hilo, South Hilo District | 2014 Nov 26 | 212 | 194 | 234 | 226 | NR | 328 | 328 | 401 | 444 |
| C. huliohia | 47143 | P15-33 | Metrosideros polymorpha | B | USA, Hawai’i, Pana’ewa, South Hilo District | 2015 Mar 3 | 212 | 194 | 234 | 226 | NR | 328 | 328 | 401 | 444 |
| C. huliohia | 47149 | P15-58 | Metrosideros polymorpha | B | USA, Hawai’i, Hōlualoa, North Kona District | 2015 Jun 10 | 212 | 194 | 234 | 226 | NR | 328 | 328 | 401 | 444 |
| C. huliohia | 46738 | P15-59 | Metrosideros polymorpha | B | USA, Hawai’i, Hōlualoa, North Kona District | 2015 Jun 10 | 212 | 194 | 234 | 226 | NR | 328 | 328 | 401 | 444 |
| C. huliohia | 46739 | P15-60 | Metrosideros polymorpha | B | USA, Hawai’i, Hōlualoa, North Kona District | 2015 Jun 10 | 212 | 194 | 234 | 226 | – | 328 | 328 | 401 | – |
| C. huliohia | 47151 | P15-62 | Metrosideros polymorpha | B | USA, Hawai’i, Hōlualoa, North Kona District | 2015 Jun 10 | 212 | 194 | 234 | 226 | – | 328 | 328 | 401 | – |
| C. uchidae | 14796 | CBS 114720 | Colocasia esculenta | - | USA, Hawai’i, O’ahu | 1991 | 219,68 | 194 | 249 | 226 | 270 | 328 | – | 401 | 444 |
| C. uchidae | 14804 | CBS 115164 | Colocasia esculenta | - | USA, Hawai’i, Kaua’i | 1988 | 219,75 | 194 | 249 | 226 | 270 | 328 | – | 401 | 444 |
| C. cercfabiensis | 43029 | CERC 2170 | Eucalyptus sp. | - | China, HaiNan Province | 2013 | 203 | 194 | 237 | 226 | 294 | 328 | – | 401 | 444 |
| C. cercfabiensis | 42795 | CERC 2687 | Eucalyptus sp. | - | China, GuangDong | 2013 | 206 | 194 | 237 | 226 | 291 | 334 | 328 | 401 | 447 |
| C. changhui | 43272 | CERC 3605 | Colocasia esculenta | - | China, YunNan Province | 2015 | 220 | 194 | 237 | 226 | 270 | 328 | – | 401 | 444 |
| C. changhui | 43281 | CERC 3615 | Colocasia esculenta | - | China, YunNan Province | 2014 | 220 | 194 | 237 | 226 | 270 | 328 | – | 401 | 444 |
| C. changhui | 49317 | C3371 | Eucalyptus sp. | - | China, YunNan Province | 2014 | 232 | 194 | 243 | 226 | 270 | 328 | 328 | 401 | 444 |
| C. changhui | 49318 | C3372 | Eucalyptus sp. | - | China, YunNan Province | 2014 | 232 | 194 | 243 | 226 | 270 | 328 | 328 | 401 | 444 |
| C. changhui | 49319 | C3373 | Eucalyptus sp. | - | China, YunNan Province | 2014 | 232 | 194 | 243 | 226 | 270 | 328 | 328 | 401 | 444 |
| C. changhui | 49320 | C3374 | Eucalyptus sp. | - | China, YunNan Province | 2014 | 232 | 194 | 243 | 226 | 270 | 328 | 328 | 401 | 444 |
| C. changhui | 49321 | C3375 | Eucalyptus sp. | - | China, YunNan Province | 2014 | 232 | 194 | 243 | 226 | 270 | 328 | 328 | 401 | 444 |
| C. changhui | 49322 | C3376 | Eucalyptus sp. | - | China, YunNan Province | 2014 | 232 | 194 | 243 | 226 | 270 | 328 | 328 | 401 | 444 |
| C. polychroma | 11424 | Syzygium aromaticum | - | Indonesia | 2002 | 209 | 194 | 234 | 226 | 294 | 328/334 | – | 401 | 444 | |
| C. polychroma | 11436 | Syzygium aromaticum | Indonesia | 2002 | 209 | 194 | 234 | 226 | 294 | 334 | – | 401 | 444 | ||
MB = multiple bands observed during PCR, did not do fragment analyses; - = no data available; – = PCR was not done; FP = finger print pattern that was difficult to score; NR = no PCR amplification; MA = multiple alleles observed.